SYSTEMATIC SCREENING OF SYNTHETIC ORGANOCHALCOGEN COMPOUNDS WITH ANTICANCER ACTIVITY USING HUMAN LUNG ADENOCARCINOMA SPHEROIDS.

Lung adenocarcinoma stands as a leading global cause of cancer-related fatalities, with current therapeutic approaches remaining unsatisfactory. Given the association between elevated oxidative markers and the aggressive nature of cancer cells (including multidrug resistance and metastatic potential) that can predict poor outcome of lung adenocarcinoma patients, any compounds that interfere with their aberrant redox biology should be rationally explored as innovative intervention strategies. This study was designed to screen potential anticancer activities within nine newly synthesized organochalcogen - compounds characterized by the presence of oxygen, sulfur, or selenium elements in their structure and exhibiting antioxidant activity - and systematically evaluated their performance against cisplatin, the cornerstone therapeutic agent for lung adenocarcinoma. Our methodology involved the establishment of optimal conditions for generating single tumor spheroids using A549 human lung adenocarcinoma cell line. The initiation interval for spheroid formation was determined to be four days in vitro (DIV), and these single spheroids demonstrated sustained growth over a period of 20 DIV. Toxic dose-response curves were subsequently performed for each compound after 24 and 48 hours of incubation at the 12nd DIV. Our findings reveal that at least two of the synthetic organochalcogen compounds exhibited noteworthy anticancer activity, surpassing cisplatin in key parameters such as lower LD (Lethal Dose) 50, larger drug activity area, and maximum amplitude of effect, and are promising drugs for futures studies in the treatment of lung adenocarcinomas. Physicochemical descriptors and prediction ADME (absorption, distribution, metabolism, and excretion) parameters of selected compounds were obtained using SwissADME computational tool; Molinspiration server was used to calculate a biological activity score, and possible molecule targets were evaluated by prediction with the SwissTargetPrediction server. This research not only sheds light on novel avenues for therapeutic exploration but also underscores the potential of synthetic organochalcogen compounds as agents with superior efficacy compared to established treatments.

BMP6 and VDR gene polymorphisms are associated with osteonecrosis in a sickle cell anaemia cohort.

The occurrence and severity of osteonecrosis in sickle cell anaemia (SCA) vary due to risk factors, including genetic modifiers. Bone morphogenetic proteins (BMPs), particularly BMP6, and the vitamin D receptor (VDR) play key roles in cartilage and bone metabolism, making them potential contributors to orthopaedic outcomes in SCA. Here, we evaluated the association of polymorphisms in BMP6 (rs3812163, rs270393 and rs449853) and VDR (FokI rs2228570 and Cdx2 rs11568820) genes with osteonecrosis risk in a Brazilian SCA cohort. A total of 177 unrelated SCA patients were selected. The AA genotype of BMP6 rs3812163 was independently associated with a lower osteonecrosis risk (p = 0.015; odds ratio (OR): 0.38; 95% confidence interval (CI): 0.18-0.83) and with the long-term cumulative incidence of osteonecrosis (p = 0.029; hazard ratio: 0.56, 95% CI: 0.34-0.94). The VDR rs2228570 TT genotype was independently associated with a lower osteonecrosis risk (p = 0.039; OR: 0.14; 95% CI: 0.02-0.90). In summary, our results provide evidence that BMP6 rs3812163 and the VDR rs2228570 might be implicated in osteonecrosis pathophysiology in SCA and might help identify individuals at high risk.

A-296G variant of THBS1 gene (rs1478605) is associated with a lower frequency of stroke in a Brazilian population with sickle cell anemia.

OBJECTIVES: Stroke is a devastating clinical outcome that significantly contributes to the morbidity and mortality of sickle cell anemia (SCA) patients. Despite its advantages in predicting stroke risk, transcranial Doppler screening has limitations that restrict its applicability, highlighting the need for emerging prognostic tools. Thrombospondin-1 plays a crucial role in endothelial injury, platelet adhesion, and nitric oxide metabolism and may be implicated in stroke pathophysiology. Here, we aimed to evaluate the association of THBS1 genetic variations with the occurrence of stroke in SCA patients MATERIALS AND METHODS: By real-time PCR, 512 SCA patients were fully genotyped for THBS1 A-296G (rs1478605) polymorphism RESULTS: THBS1 GG genotype was associated with a lower risk for stroke occurrence [odds ratio (OR): 0.30; 95% confidence interval (CI): 0.11-0.78; P = 0.011], although these findings were not consistent with multivariate logistic regression analysis (OR: 0.73, 95% CI: 0.12 - 4.37; P = 0.736). In agreement, the cumulative incidence of stroke for patients with AG/AA genotypes was higher when compared to the GG genotype (P = 0.018). However, the association was not maintained in the multivariate proportional hazards model (hazard ratio: 0.67, 95% CI: 0.12-3.61; P = 0.643) CONCLUSIONS: In summary, the present study shows that the THBS1 A-296G (rs1478605) polymorphism may be a potential modifier for stroke in SCA.

Perfil funcional de pessoas com dores persistentes na coluna baseado na Classificação Internacional de Funcionalidade / Functioning profile of people with persistent back pain based on International Classification of Functioning

Traçar o perfil de funcionalidade de pessoas com dores persistentes na coluna por meio da Classificação Internacional de Funcionalidade, Incapacidade e Saúde (CIF). Trata-se de um estudo transversal com aplicação do método SCEBS (Somatic, Cognition, Emotion, Behavior and Social), Escala de catastrofização da dor, escala de cinesiofobia e percepção corporal. O discurso dos participantes foi analisado para identificação de conteúdos da CIF. O estudo incluiu 49 participantes com média de 25 anos. A partir do discurso foram identificadas 2.053 citações relacionadas a 108 diferentes categorias da CIF. As categorias mais identificadas foram: segmento vertebral lombar (s76002), funções relacionadas aos aspectos emocionais (b1522 e b152), cuidados da própria saúde (d570) e educação (d838). A catastrofização e cinesiofobia interferiram no perfil de funcionalidade dos participantes. As pessoas com dor persistente na coluna enfrentam prejuízos nas estruturas e funções corporais, limitação nas atividades e participação, fatores ambientais e pessoais.

To outline the functional profiles of individuals with persistent back pain using the International Classification of Functioning, Disability, and Health (ICF). This cross-sectional study employed the Somatic, Cognition, Emotion, Behavior, and Social (SCEBS) method, the Pain Catastrophizing Scale, the Kinesiophobia Scale, and body perception measures. The participants' discourses were analyzed to identify the content related to the ICF. This study included 49 participants with an average age of 25 years. From the discourse analysis, 2,053 citations related to 108 ICF categories were identified. The most frequently identified categories were the lumbar vertebral column (s76002), functions related to emotional aspects (b1522 and b152), self-care (d570), and education (d838). Catastrophizing and kinesiophobia influenced participants' functional profiles. Individuals with persistent back pain experience impairments in body structure and function, limitations in activities and participation, and environmental and personal factors.

Microextraction by packed sorbent and high-performance liquid chromatography for determination of benznidazole in human plasma.

Benznidazole is the main drug used in Chagas disease and its determination in plasma samples is useful in several situations. Hence, robust and accurate bioanalytical methods are needed. In this context, sample preparation deserves special attention, as it is the most error-prone, labor-intensive and time-consuming step. Microextraction by packed sorbent (MEPS) is a miniaturized technique, developed to minimize the use of hazardous solvents and sample amount. In this context, this study aimed to develop and validate a MEPS coupled to high performance liquid chromatography method for the analysis of benznidazole in human plasma. MEPS optimization was performed by a 24 full factorial experimental design, which resulted in about 25 % of recovery. The best condition was achieved when 500 µL of plasma,10 draw-eject cycles, sample volume drawn of 100 µL, and desorption with three times of 50 µL of acetonitrile were used. The chromatographic separation was performed with a C18 (150 × 4.5 mm, 5 µm) column. The mobile phase was composed of water:acetonitrile (60:40) at a flow rate of 1.0 mL min-1. The developed method was validated and proved to be selective, precise, accurate, robust and linear in the range from 0.5 to 6.0 µg mL-1. The method was applied to three healthy volunteers that made use of benznidazole tablets and showed to be adequate to assess this drug in plasma samples.

Boron nitride nanotubes for extraction of angiotensin receptor blockers from human plasma.

The limitations of silica-based sorbents boosted the development of new extraction phases. In this study, boron nitride nanotubes functionalized with octadecyl groups were used for the first time as sorbent for extraction of losartan and valsartan, the most used angiotensin receptor blockers in the clinical practice, from human plasma. The nanotubes were synthesized using the chemical vapor deposition technique, purified by acid treatment, functionalized with octadecylamine in a microwave reactor, and characterized by different techniques. The functionalized nanotubes were packed in solid phase extraction cartridges. Extraction conditions were optimized by means of a 23 factorial design with center points. The separation was performed on a biphenyl core-shell (100 × 4.6 mm; 2.6 µm) column, using 0.1 % (v/v) triethylamine solution and methanol (pH 3.2) as mobile phase, at 0.7 mL/min, in gradient elution. The injection volume was 10 µL and fluorescence detection was performed at excitation and emission wavelengths of 250 and 375 nm, respectively. The developed method was validated according to Brazilian Health Regulatory Agency (ANVISA), United States Food and Drug Administration (US FDA) and European Medicines Agency (EMA) guidelines and presented selectivity, precision, accuracy, and linearity in the concentration ranges of 50-1200 ng/mL for losartan and 20-1700 ng/mL for valsartan. Recoveries higher than 80 % were obtained. The method was fit for the quantification of losartan in plasma samples from patients under antihypertensive therapy, being useful in therapeutic drug monitoring, pharmacokinetics and bioequivalence studies.

Quality of sleep and musculoskeletal pain in adolescents: observational study / Qualidade de sono e dores musculoesqueléticas em adolescentes: estudo observacional

ABSTRACT BACKGROUND AND OBJECTIVES: Musculoskeletal pain (MSP) is related to psychogenic factors and quality of sleep, showing that this triad is a biopsychosocial process. The aim of this study was to analyze an association between poor sleep quality and MSP in adolescents. METHODS: This study has an observational, cross-sectional character, in which 545 adolescents, aged between 11 and 15 years, were analyzed. Sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI), the presence of MSP using the Nordic Musculoskeletal Questionnaire (NMQ), temporomandibular disorder using the Axis II of the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) and excessive daytime sleepiness using the Epworth Sleepiness Scale (ESS). Pearson Chi-square or Fishers Exact test were used to assess the association between two categorical variables. RESULTS: The prevalence of poor sleep quality, sleep disorders, MSP and excessive daytime sleepiness were, respectively, 66.8, 9.5, 87.5 and 30.5%. An association was observed between the quality of sleep and the number of hours of sleep per night [OR = 1.49; (1.01 to 2.21)], and between sleep disorders and MSP in the upper back [OR=1.9; (1.0 to 3.3)], and the wrists and hands [OR=2.8; (1.4 to 5.7)]. However, there was no association between sleep quality and MSP [OR=1.29; (0.76 to 2.17)]. CONCLUSION: An association was found between sleep disorders and MSP, as well as between the number of hours slept per night and quality of sleep.

RESUMO JUSTIFICATIVA E OBJETIVOS: As dores musculoesqueléticas (DME) possuem relação com fatores psicogênicos e qualidade de sono, evidenciando que essa tríade se refere a um processo biopsicossocial. O objetivo deste estudo foi analisar a associação entre má qualidade de sono e DME em adolescentes. MÉTODOS: Realizou-se estudo observacional, com delineamento transversal, no qual foram analisados 545 adolescentes, na faixa etária entre 11 e 15 anos. A qualidade do sono foi avaliada através do Índice de Qualidade do Sono de Pittsburgh (PSQI), a presença de DME pelo Questionário Nórdico de Sintomas Osteomusculares (QNSO), disfunções temporomandibulares pelo Eixo II dos Critérios Diagnósticos de Pesquisa em Disfunção Temporomandibular (RDC/TMD) e a sonolência diurna excessiva através da Escala de Sonolência de Epworth (ESE). Para avaliar associação entre duas variáveis categóricas, foi utilizado o teste Qui-quadrado de Pearson ou o teste Exato de Fisher. RESULTADOS: A prevalência da má qualidade do sono, distúrbios do sono, DME e sonolência diurna excessiva foram 66,8, 9,5, 87,5 e 30,5%, respectivamente. Observou-se associação entre qualidade do sono e quantidade de horas de sono por noite [OR=1,49; (1,01 a 2,21)], e entre distúrbios do sono e DME na parte superior das costas [OR=1,9; (1,0 a 3,3)] e nos punhos e mãos [OR=2,8; (1,4 a 5,7)]. No entanto, não foi verificada associação entre qualidade do sono e DME [OR=1,29; (0,76 a 2,17)]. CONCLUSÃO: Foi verificada associação entre distúrbios de sono e DME, bem como entre qualidade do sono e quantidade de horas dormidas por noite.

Silver nanoparticle incorporation into flexible polyamide 12 membranes.

To meet the demands of the market and society, the development of structured polymeric materials for application in the medical field is constantly increasing. Over the last decades, metallic silver nanoparticles have been explored due to their antimicrobial action. Here, we aimed to incorporate metallic silver nanoparticles into polymeric pieces obtained by additive manufacture via a chemical route involving silver nitrate and sodium borohydride. Polyamide 12 membranes were obtained by selective laser sintering, which was followed by washing, pretreatment, and functionalization with the alkoxides tetraethylorthosilicate and 3-aminopropyl tetraethoxysilane. For nanoparticle preparation and incorporation, a chemical route was tested under different conditions. The samples were characterized by techniques, such as X-ray diffraction, ultraviolet-visible spectroscopy, and infrared vibrational spectroscopy. Nanoparticle formation and incorporation into the polyamide 12 membranes were demonstrated by the absorbance band at 420 nm, which indicated that the particles measured between 10 and 50 nm in size; by the X-ray diffraction peaks at 2θ = 38, 44, and 64°, which are typical of crystalline silver; and by vibrational spectroscopy, which evidenced that the nanoparticles interacted with the polyamide 12 nitrogen groups. Polyamide 12 membranes containing metallic silver nanoparticles have promising biomedical applications as antimicrobial wound dressings associated with drug carriers.

Are exercises with or without occlusal splints more effective in the reduction of pain in patients with temporomandibular disorders of myogenic origin? A systematic review

Abstract Temporomandibular disorders (TMD) is a term used to describe a set of clinical conditions that may compromise the temporomandibular joint (TMJ) and masticatory muscles and/or associated structures, considered the most frequent cause of orofacial pain of non-dental origin. In recent years, many forms of physical therapy have been used in the treatment of TMD to reduce pain and improve the range of mandibular movement present in this impairment. Among these resources are kinesiotherapy (exercise), electrothermal and manual therapy, acupuncture, training posture, mobilizations, and biofeedback. Objectives To determine if exercises with or without occlusal splints are effective in reducing pain in patients with temporomandibular disorders (TMD) of myogenic origin. Methodology This systematic review was registered in the International Prospective Register of Systematic Reviews (CRD 42019134244). Controlled trials published in PubMed, Scopus, and Cochrane Library following PRISMA guidelines up to April 2022 were randomized and included. The population above 18 years, which evaluated the effectiveness of exercise with or without occlusal splints in reducing pain in patients with TMD of myogenic origin, diagnosed through the Research Diagnostic Criteria for Temporomandibular Disorders, was also included. There was no restriction on the period of publication. Cochrane risk of bias analysis was performed. Results Of the five included articles, all showed a reduction of pain, but without significant differences between the interventions performed. Additionally, studies that evaluated the quality of life and mandibular movements showed a reduction in pain, but no significant differences between therapies. Conclusion The analyzed studies showed no difference in the improvement of pain, quality of life, and mandibular movements between the groups that performed only exercises or the associated treatments.

Association of KLOTHO polymorphisms with clinical complications of sickle cell anemia.

The clinical and phenotypic heterogeneity of patients with sickle cell anemia (SCA) is influenced by environmental and genetic factors. Several genetic modifiers, such as the KLOTHO (KL) gene, have been associated with SCA clinical outcomes. The KL gene and its encoded proteins are implicated in important biological pathways, which affect the disease's pathophysiology, such as expression of adhesion molecules VCAM-1 and ICAM-1, oxidative stress, and nitric oxide biology. Here, we evaluated the clinical relevance of two polymorphisms found on the KL gene (rs685417 and rs211239) in 588 unrelated patients with SCA. Genotyping analyses were performed using the TaqMan system. The KL rs211239 was associated with increased number of vaso-occlusive crisis (VOCs) per year (P = 0.001), while KL rs685417 was associated with increased frequency of stroke (P = 0.034), priapism (P = 0.011), number of complications (P = 0.019), and with a lower incidence of priapism (P = 0.036). Additionally, the associations with VOCs, stroke, and priapism remained consistent in multivariate analyses (P < 0.05). Our data highlight the clinical importance of KL in SCA.

Primary cutaneous histoplasmosis difficult to treat in immunocompetent patient: case report and literature review.

Histoplasmosis is an infection caused by the dimorphic fungus Histoplasma capsulatum. The disease is endemic in several regions of tropical and temperate climate. The fungus presents opportunistic behavior, causing widespread infection in immunocompromised patients, resulting from complication of primary pulmonary infection, due to exogenous reinfection or reactivation of a quiescent source. In immunocompetent individuals, approximately 95% of pulmonary infections are asymptomatic. However, prolonged exposure to high amount spores may lead to acute or chronic lung infection. Due to the low amount of inoculum, primary cutaneous histoplasmosis caused by traumatic implantation is extremely rare and effectively treated with triazoles. Thus, the present study aims to report a case of primary cutaneous histoplasmosis that is difficult to treat in an immunocompetent patient, and to review the literature on the incidence of drug-resistant Histoplasma capsulatum strains in clinical practice.

Alpha thalassemia, but not ßS-globin haplotypes, influence sickle cell anemia clinical outcome in a large, single-center Brazilian cohort.

Alpha thalassemia and beta-globin haplotype are considered classical genetic disease modifiers in sickle cell anemia (SCA) causing clinical heterogeneity. Nevertheless, their functional impact on SCA disease emergence and progression remains elusive. To better understand the role of alpha thalassemia and beta-globin haplotype in SCA, we performed a retrospective study evaluating the clinical manifestations of 614 patients. The univariate analysis showed that the presence of alpha-thalassemia -3.7-kb mutation (αα/-α and -α/-α) decreased the risk of stroke development (p = 0.046), priapism (p = 0.033), and cholelithiasis (p = 0.021). Furthermore, the cumulative incidence of stroke (p = 0.023) and cholelithiasis (p = 0.006) was also significantly lower for patients carrying the alpha thalassemia -3.7-kb mutation. No clinical effects were associated with the beta-globin haplotype analysis, which could be explained by the relatively homogeneous haplotype composition in our cohort. Our results reinforce that alpha thalassemia can provide protective functions against hemolysis-related symptoms in SCA. Although, several genetic modifiers can impact the inflammatory state of SCA patients, the alpha thalassemia mutation remains one of the most recurrent genetic aberration and should therefore always be considered first.

Influence of UGT1A1 promoter polymorphism, α-thalassemia and ßs haplotype in bilirubin levels and cholelithiasis in a large sickle cell anemia cohort.

Hyperbilirubinemia in patients with sickle cell anemia (SCA) as a result of enhanced erythrocyte destruction, lead to cholelithiasis development in a subset of patients. Evidence suggests that hyperbilirubinemia may be related to genetic variations, such as the UGT1A1 gene promoter polymorphism, which causes Gilbert syndrome (GS). Here, we aimed to determine the frequencies of UGT1A1 promoter alleles, alpha thalassemia, and ßS haplotypes and analyze their association with cholelithiasis and bilirubin levels. The UGT1A1 alleles, -3.7 kb alpha thalassemia deletion and ßS haplotypes were determined using DNA sequencing and PCR-based assays in 913 patients with SCA. The mean of total and unconjugated bilirubin and the frequency of cholelithiasis in GS patients were higher when compared to those without this condition, regardless of age (P < 0.05). Cumulative analysis demonstrated an early age-at-onset for cholelithiasis in GS genotypes (P < 0.05). Low fetal hemoglobin (HbF) levels and normal alpha thalassemia genotype were related to cholelithiasis development (P > 0.05). However, not cholelithiasis but total and unconjugated bilirubin levels were associated with ßS haplotype. These findings confirm in a large cohort that the UGT1A1 polymorphism influences cholelithiasis and hyperbilirubinemia in SCA. HbF and alpha thalassemia also appear as modulators for cholelithiasis risk.

Primary cutaneous histoplasmosis difficult to treat in immunocompetent patient: case report and literature review / Histoplasmose cutânea primária de difícil tratamento em paciente imunocompetente: relato de caso e revisão da literatura

ABSTRACT Histoplasmosis is an infection caused by the dimorphic fungus Histoplasma capsulatum. The disease is endemic in several regions of tropical and temperate climate. The fungus presents opportunistic behavior, causing widespread infection in immunocompromised patients, resulting from complication of primary pulmonary infection, due to exogenous reinfection or reactivation of a quiescent source. In immunocompetent individuals, approximately 95% of pulmonary infections are asymptomatic. However, prolonged exposure to high amount spores may lead to acute or chronic lung infection. Due to the low amount of inoculum, primary cutaneous histoplasmosis caused by traumatic implantation is extremely rare and effectively treated with triazoles. Thus, the present study aims to report a case of primary cutaneous histoplasmosis that is difficult to treat in an immunocompetent patient, and to review the literature on the incidence of drug-resistant Histoplasma capsulatum strains in clinical practice.

RESUMO A histoplasmose é uma infecção causada pelo fungo dimórfico Histoplasma capsulatum. A doença é endêmica em diversas regiões de clima tropical e temperado. O fungo apresenta comportamento oportunístico, causando infecção disseminada em pacientes imunocomprometidos, resultante da complicação da infecção pulmonar primária, por reinfecção exógena ou reativação de um foco quiescente. Em indivíduos imunocompetentes, cerca de 95% das infecções pulmonares são assintomáticas. No entanto, a exposição prolongada à quantidade elevada de esporos pode levar à infecção pulmonar aguda ou crônica. Devido à baixa quantidade de inóculo, a histoplasmose cutânea primária causada por implantação traumática é extremamente rara e efetivamente tratada com triazóis. Assim, o presente estudo tem como objetivos relatar um caso de histoplasmose cutânea primária de difícil tratamento em paciente imunocompetente, e revisar a literatura a respeito da incidência de cepas de Histoplasma capsulatum resistentes aos fármacos utilizados na prática clínica.

Comparison of factors associated with leukemia and lymphoma mortality in Brazil.

In the last decades, few epidemiological studies have discussed the mortality rates due to leukemia and lymphoma in Brazil. This study analyzes the evolution over time of the number of deaths due to leukemia and lymphoma in Brazil, between 2010 and 2016, considering the population's characteristics and spatial distribution. This is a retrospective epidemiological study based on data obtained in the Brazilian Health Informatics Department (DATASUS), associated with the quantitative population. We created choropleth maps and predictive models of mortality rates, using the incidence rate ratio (IRR) to measure the size of the effect. Leukemia had a 1.76 higher mortality rate than lymphoma. Leukemia mortality trends increased by 1.2% per year between 2010 and 2016. Regions with the lowest social inequality had higher mortality rates for both diseases. There was a difference between peaks with higher chances of death due to leukemia (> 60 years) and lymphoma (> 70 years). Older age, male, white, and South and Southeast regions were associated with higher mortality by leukemia or lymphoma.

Sleep disturbance and depression in adolescence: an integrative review of literature.

This study investigated the association between sleep disorders and depression in adolescents, PubMed/MedLine, SciELO and Lilacs databases were consulted, with an interval of 7 years (2013-2019), in English, Portuguese and Spanish. An integrative review was carried out where after analyzing the inclusion criteria, 616 articles were selected for reading titles and abstracts and 94 of these were selected for full reading. After the full reading, 15 articles were elected to be part of this review. Results show that both sleep disorders are risk factors for depression, as depression can be a risk factor for sleep disorders. Insomnia was the most frequent sleep disorder in adolescence, besides being considered a public health problem. It is concluded that sleep disorders and depressive symptoms are associated and present with great frequency in the adolescent public.

Comparison of factors associated with leukemia and lymphoma mortality in Brazil / Comparação de fatores associados com a mortalidade por leucemia e linfoma no Brasil / Comparación de factores asociados a la mortalidad por leucemia y linfoma en Brasil

Abstract: In the last decades, few epidemiological studies have discussed the mortality rates due to leukemia and lymphoma in Brazil. This study analyzes the evolution over time of the number of deaths due to leukemia and lymphoma in Brazil, between 2010 and 2016, considering the population's characteristics and spatial distribution. This is a retrospective epidemiological study based on data obtained in the Brazilian Health Informatics Department (DATASUS), associated with the quantitative population. We created choropleth maps and predictive models of mortality rates, using the incidence rate ratio (IRR) to measure the size of the effect. Leukemia had a 1.76 higher mortality rate than lymphoma. Leukemia mortality trends increased by 1.2% per year between 2010 and 2016. Regions with the lowest social inequality had higher mortality rates for both diseases. There was a difference between peaks with higher chances of death due to leukemia (> 60 years) and lymphoma (> 70 years). Older age, male, white, and South and Southeast regions were associated with higher mortality by leukemia or lymphoma.

Resumo: Nas últimas décadas, poucos estudos epidemiológicos examinaram as taxas de mortalidade por leucemia e linfoma no Brasil. O presente estudo faz uma análise retrospectiva da evolução temporal do número de óbitos por leucemia e linfoma no Brasil entre 2010 e 2016, considerando as características e a distribuição espacial da população. O estudo epidemiológico utilizou dados do Departamento de Informática do Sistema Único de Saúde (DATASUS), associados aos dados quantitativos da população. Foram elaborados mapas coropléticos e modelos preditivos de taxas de mortalidade. Utilizamos a razão de taxas de incidência (RTI) como medida do tamanho do efeito. A leucemia mostrou uma taxa de mortalidade 1,76 vez mais elevada que os linfomas. A mortalidade por leucemia aumentou 1,2% por ano entre 2010 e 2016. As regiões com menor desigualdade social mostraram taxas de mortalidade mais altas para ambas doenças. Houve uma diferença entre os picos, com chances mais altas de morrer por leucemia (> 60 anos) e por linfoma (> 70 anos). Idade mais avançada, sexo masculino, cor branca e regiões Sul e Sudeste estiveram associados a taxas de mortalidade mais altas por leucemia ou linfoma.

Resumen: En las últimas décadas, pocos estudios epidemiológicos han analizado las tasas de mortalidad causadas por leucemia y linfoma en Brasil. Este estudio analiza, retrospectivamente, la evolución temporal del número de muertes, debidas a la leucemia y linfoma en Brasil, entre 2010 y 2016, considerando las características de la población y su distribución espacial. Este es un estudio epidemiológico, basado en los datos obtenidos del Departamento de Informática del Sistema Único de Salud (DATASUS), asociado con población cuantitativa. Se elaboraron mapas coropléticos y modelos predictivos de tasas de mortalidad. Usamos la razón de tasas de incidencia (RTI) como medida del tamaño del efecto. La leucemia tuvo una tasa de mortalidad un 1,76 mayor que los linfomas. Las tendencias de mortalidad por leucemia se incrementaron en un 1,2% al año entre 2010 and 2016. Las regiones con la desigualdad social más baja contaron con unas tasas de mortalidad más altas en ambas enfermedades. Había una diferencia entre los picos con mayores oportunidades de muerte debido a la leucemia (> 60 años) y linfoma (> 70 años). Contar con una avanzada edad, género masculino, autodeclarado blanco y procedente de las regiones Sur y Sudeste estuvieron relacionados con una mortalidad más alta a causa de leucemia o linfoma.

Avaliação sensorial de urucum: aceitabilidade e expectativa das marcas comerciais / Sensory evaluation of urucum: acceptability and expectation of brands

O consumo de condimentos é cada vez mais usual, principalmente no que diz respeito aos corantes naturais. O urucum faz parte desta categoria, porém poucos estudos são voltados a sua aceitabilidade e avaliação sensorial, com isso, objetivou-se avaliar marcas comerciais de coloríficos de urucum e o efeito da intensidade da cor e do aroma, e a aceitação dos atributos cor, aparência e aceitação global, além da atitude de compra e análise da embalagem. Foram analisadas 6 marcas comerciais por 63 julgadores consumidores de coloríficos. Os resultados demonstram que, houve variação das notas atribuídas pelos julgadores as marcas comerciais, e que a escolha dos julgadores é influenciada pelo tipo de embalagem utilizada no acondicionamento dos coloríficos.

Population fluctuations in the pink hibiscus mealybug and its natural enemies in Annona squamosa (Annonaceae) in Roraima, Brazil / Flutuação populacional da cochonilha-rosada e seus inimigos naturais em Annona squamosa (Annonaceae) em Roraima, Brasil

Maconellicoccus hirsutus (Hemiptera, Pseudococcidae), a species of economic interest, especially for fruit plants, is expanding on the South American continent. Information about the population dynamics of this pest associated with control by natural enemies and cultural practices is fundamental for its management. Our objective was to study the population fluctuations in M. hirsutus and its natural enemies in a sugar-apple (Annona squamosa) orchard in Roraima, northern Brazil. Trees were evaluated monthly over a 12-month period. Infestation rates by M. hirsutus and its parasitism were also estimated for potential host plants around the study area. Highest infestation occurred in August and February-March. Alternative hosts were infested during the off-season, mainly fruit. Lacewings and the parasitoid Anagyrus kamali (Hymenoptera, Encyrtidae) were abundant natural enemies. Average parasitism by A. kamali in fruits was 50%, with highest rates in periods of greatest infestation by M. hirsutus. Fruitification pruning reduced M. hirsutus populations.

Maconellicoccus hirsutus (Hemiptera, Pseudococcidae), praga de inúmeras espécies, especialmente frutíferas, está se expandindo no continente sul-americano. Informações sobre sua dinâmica, inimigos naturais e práticas culturais são importantes para seu manejo. Nosso objetivo foi estudar as flutuações da população em M. hirsutus e seus inimigos naturais em um pomar de ata (Annona squamosa) em Roraima, Brasil. As árvores foram avaliadas mensalmente ao longo de um período de 12 meses e as taxas de infestação por M. hirsutus e parasitismo foram estimadas. A maior infestação ocorreu em agosto de 2014 e entre fevereiro e março de 2015. Hospedeiros alternativos foram infestados durante a entressafra da ata. Bicho-lixeiro e o parasitoide Anagyrus kamali (Hymenoptera, Encyrtidae) foram os inimigos naturais mais importantes. O parasitismo médio por A. kamali nos frutos foi de 50%, com maiores taxas nos períodos de maior infestação por M. hirsutus. A poda de frutificação reduziu as populações de M. hirsutus.